BBa_I746100
BBa_I746100 Version 1 (Component)
AIP sensor infrastructure (agrC + agrA from S. aureus with RBSes)

BBa_I746101
BBa_I746101 Version 1 (Component)
I746100 (AIP sensor infrastructure - agrC + agrA with RBSes) + B0015 (Terminator)

BBa_I746103
BBa_I746103 Version 1 (Component)
I746101 (AIP sensor infrastructure - agrC + agrA with RBSes + Terminator) + I746104 (Inducible P2)

BBa_I746107
BBa_I746107 Version 1 (Component)
I746101 (AIP sensor infrastructure) + I746105 (Inducible P2 + GFP reporter)

BBa_M39023
BBa_M39023 Version 1 (Component)
AND gate structure for regulation of E.coli in CF mucus

BBa_K772003
BBa_K772003 Version 1 (Component)
TEV protease Full Structure

BBa_K1314018
BBa_K1314018 Version 1 (Component)
nif structure 5 in pUC57 (amp resistance)

O2
BBa_K2054002 Version 1 (Component)
Oligo 2 of DNA Tetrahedral Nanostructure

O3
BBa_K2054003 Version 1 (Component)
Oligo 3 of DNA Tetrahedral Nanostructure

O4
BBa_K2054004 Version 1 (Component)
Oligo 4 of DNA Tetrahedral Nanostructure

O5
BBa_K2054005 Version 1 (Component)
Oligo 5 of DNA Tetrahedral Nanostructure

Tetrahedro
BBa_K2054000 Version 1 (Component)
All Oligos for Tetrahedral Nanostructure

Active Com
BBa_K2054006 Version 1 (Component)
All Oligos for simplified Tetrahedral Nanostructure

BBa_K897318
BBa_K897318 Version 1 (Component)
Paired termini structure

TBY
BBa_K897720 Version 1 (Component)
Antisense FtsZ protected by paired termini structure under T7 promoter

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.